Experiment with pseudo-replication
To perform an experiment a tad simpler, it might seem best to the researcher to just apply a treatment to all the trees in a row, and then the next treatment to all the trees in the next row, etc. The experiment is being replicated isn't it by the number of trees in each row?
Unfortunately, this experiment cannot be legitmately analysed because the null hypothesis cannot be tested. If treatments are applied to whole rows, then treatment should be applied to replicate rows. One such design, the split-block, is used in such circumstances.
Row I A A A A Row II B B B B Row III C C C C Row IV D D D D
ANOVA table format:
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